Towards Single Cell Fingerprinting in Microfluidic Device Format: Single Cell Manipulation, Protein Separation and Detection
نویسندگان
چکیده
Our single cell analytical experiments for future protein fingerprinting combines microfluidic on-chip protein electrophoresis with latest laser technology for single cell manipulation and (label-free) protein detection, and is presented by the following issues: 1) Single cells were trapped and navigated by optical tweezers (OT) in a PDMS microfluidic device, and lysed at a predefined position. 2) Separation and detection of proteins was achieved with laser induced fluorescence (LIF) detection in the visible (488 nm) as well as in the UV (266 nm) spectral range for label-free protein detection. 3) First successful experiments with single GFP-transfected Sf9 insect cells exhibit distinct single component electropherograms.
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Single cell manipulation, analytics, and label-free protein detection in microfluidic devices for systems nanobiology.
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